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Antifungal agents from heartwood extract of Taiwania cryptomerioides against brown root rot fungus Phellinus noxius
- Source :
- Wood Science and Technology. 51:639-651
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Taiwania (Taiwania cryptomerioides Hayata) is a native and plantation species in Taiwan, and its heartwood has demonstrated excellent decay resistance. Brown root rot fungus Phellinus noxius has caused severe damage to more than 100 tree species in Taiwan. The aims of this study were to investigate the antifungal activities of Taiwania heartwood extracts against P. noxius and to identify the antifungal compounds. Antifungal activities were examined using the agar dilution method (a direct contact assay) and the inverted lid method (a vapor contact assay). Active compounds were purified using liquid–liquid partition, open-column chromatography and HPLC. Among four soluble fractions (n-hexane, ethyl acetate, n-butanol and water soluble fractions), results showed that n-hexane soluble fraction (HSF) can significantly inhibit the growth of P. noxius via the agar dilution method. Moreover, active compounds present in HSF were obtained using antifungal assay-guided fractionation. Results showed that ferruginol, T-cadinol, α-cadinol and T-muurolol were found to exhibit excellent antifungal activities against P. noxius, and their IC50 values were 16.9, 25.8, 33.8 and 50.6 μg/mL, respectively. According to the current findings, phytochemicals (ferruginol, T-cadinol, α-cadinol and T-muurolol) of Taiwania heartwood had the potential to be used as environmentally benign antifungal agents against P. noxius in place of synthetic or inorganic fungicides.
- Subjects :
- 0106 biological sciences
Phellinus noxius
Chromatography
biology
Taiwania
Ethyl acetate
Forestry
Plant Science
Fungus
biology.organism_classification
01 natural sciences
Industrial and Manufacturing Engineering
Fungicide
Ferruginol
chemistry.chemical_compound
medicine.drug_formulation_ingredient
Triadimefon
chemistry
010608 biotechnology
Botany
medicine
Root rot
General Materials Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14325225 and 00437719
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Wood Science and Technology
- Accession number :
- edsair.doi...........66f8f04f22224342682fe48bcd93bdf2