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MO271: A Single Dose of Lithium Protects Against Renal Injury Following Bilateral Ureteral Obstruction

Authors :
Maria Heloisa Massola Shimizu
Rildo Aparecido Volpini
Ana Carolina de Braganca
Mariana Moura Nascimento
Desiree Bernardo
Daniele Canale
Antonio Carlos Seguro
Source :
Nephrology Dialysis Transplantation. 37
Publication Year :
2022
Publisher :
Oxford University Press (OUP), 2022.

Abstract

BACKGROUND AND AIMS Obstructive nephropathy decreases renal blood flow (RBF) and glomerular filtration rate (GFR). Glycogen synthase kinase 3β (GSK3 β), an apoptotic enzyme, is increased in a model of unilateral obstruction. Treatment with a single dose of lithium, a selective inhibitor of GSK3β, accelerated recovery of renal function in models of cisplatin and ischemia-reperfusion induced acute kidney injury (AKI). The aim of this study was to evaluate the efficacy of a single dose of lithium in the treatment of AKI induced by bilateral ureteral obstruction (BUO) METHOD Adult male Wistar rats were divided in four groups: (i) sham (sham operated); (ii) lithium: rats treated with lithium chloride 80 mg/Kg BW Intraperitonially (IP); (iii) BUO during 24 h; (iv) BUO during 24 h and treated with the same dose immediately after ureteral desobstruction. The rats were anesthetized 48 h after desobstruction to measure inulin clearance and renal blood flow. At the end of the clearance experiments, the rats were euthanized and the kidneys were removed to measure GSK3 β renal expression. RESULTS As described in the Table 1, BUO showed markedly reduction of GFR and RBF associated with an increase of GSK3β. A single dose of lithium administration ameliorated all these alterations. CONCLUSION Lithium treatment attenuated renal dysfunction in this model of BUO-associated AKI by improving inulin clearance. These therapeutic effects were due to an inhibition of renal expression of GSK3β. This may be a new therapeutic approach for BUO-induced AKI.

Subjects

Subjects :
Transplantation
Nephrology

Details

ISSN :
14602385 and 09310509
Volume :
37
Database :
OpenAIRE
Journal :
Nephrology Dialysis Transplantation
Accession number :
edsair.doi...........677ef252c14eda6551b0b4666b63b0fa
Full Text :
https://doi.org/10.1093/ndt/gfac067.070