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Single-nucleotide and long-patch base excision repair of DNA damage in plants

Authors :
Dolores Córdoba-Cañero
Rafael R. Ariza
Teresa Roldán-Arjona
Teresa Morales-Ruiz
Source :
The Plant Journal. 60:716-728
Publication Year :
2009
Publisher :
Wiley, 2009.

Abstract

Base excision repair (BER) is a critical pathway in cellular defense against endogenous or exogenous DNA damage. This elaborate multistep process is initiated by DNA glycosylases that excise the damaged base, and continues through the concerted action of additional proteins that finally restore DNA to the unmodified state. BER has been subject to detailed biochemical analysis in bacteria, yeast and animals, mainly through in vitro reproduction of the entire repair reaction in cell-free extracts. However, an understanding of this repair pathway in plants has consistently lagged behind. We report the extension of BER biochemical analysis to plants, using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro. We have used this system to demonstrate that Arabidopsis cell extracts contain the enzymatic machinery required to completely repair ubiquitous DNA lesions, such as uracil and abasic (AP) sites. Our results reveal that AP sites generated after uracil excision are processed both by AP endonucleases and AP lyases, generating either 5'- or 3'-blocked ends, respectively. We have also found that gap filling and ligation may proceed either through insertion of just one nucleotide (short-patch BER) or several nucleotides (long-patch BER). This experimental system should prove useful in the biochemical and genetic dissection of BER in plants, and contribute to provide a broader picture of the evolution and biological relevance of DNA repair pathways.

Details

ISSN :
09607412
Volume :
60
Database :
OpenAIRE
Journal :
The Plant Journal
Accession number :
edsair.doi...........67a8fe6fe1dd3548c5074fd008b61c64
Full Text :
https://doi.org/10.1111/j.1365-313x.2009.03994.x