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Toxic Effects of Metal Oxide Nanoparticles Based on the Enzymatic Activity and Biosynthesis of β-Galactosidase Using a Mutant Strain of E. coli
- Source :
- Journal of Nanoscience and Nanotechnology. 20:1440-1446
- Publication Year :
- 2020
- Publisher :
- American Scientific Publishers, 2020.
-
Abstract
- The effects of six metal oxide nanoparticles (MO-NPs) on the activity and biosynthesis of an enzyme (β-galactosidase) were examined using a mutant strain of E. coli. Different sensitivities were observed according to the type of NP and metabolic process. The toxic effects on enzyme activity were significantly greater than on biosynthesis (p < 0.011), except in the presence of NiO. In both cases, ZnO NP caused the greatest inhibition among the tested NPs, followed by CuO. The EC50s for ZnO were 0.19 and 3.68 mg/L for enzyme activity and biosynthesis, respectively. Similar orders of toxicity were observed as follows: ZnO > CuO > NiO > Co3O4 > TiO2, Al2O3 for enzyme activity; and ZnO > CuO > NiO ≫ Al2O3, TiO2, Co3O4 for the biosynthetic process. More systematic research, including in-depth studies like investigation of the molecular mechanisms, is necessary to elucidate the detailed mechanisms of inhibition involved in both metabolic processes.
- Subjects :
- chemistry.chemical_classification
Materials science
biology
Non-blocking I/O
Biomedical Engineering
Bioengineering
General Chemistry
Metal oxide nanoparticles
Condensed Matter Physics
Enzyme assay
chemistry.chemical_compound
Enzyme
Biochemistry
chemistry
Biosynthesis
Mutant strain
Biosynthetic process
biology.protein
General Materials Science
Metabolic Process
Subjects
Details
- ISSN :
- 15334880
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Journal of Nanoscience and Nanotechnology
- Accession number :
- edsair.doi...........67b355b2d88527a9081890f190b0b387