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Stimulated emission on microscopic scale: Light quenching of Pyridine 2 using a Ti:sapphire laser

Authors :
Karsten Bahlmann
Joseph R. Lakowicz
Martin Schrader
F. Meinecke
Stefan W. Hell
Ignacy Gryczynski
Source :
Journal of Microscopy. 180:RP1-RP2
Publication Year :
1995
Publisher :
Wiley, 1995.

Abstract

Summary We report the realization of stimulated emission on a microscopic scale. An experiment is presented describing significant depopulation of the excited state of the fluorophore Pyridine 2 with a mode-locked Ti: Sapphire laser. Stimulated emission is performed at 750 nm and excitation at the frequency doubled wavelength of 375 nm. The pulses are synchronized so that the stimulating pulse follows the excitation pulse after 5 ps. The set-up is a modified 4Pi-confocal microscope employing one of the objective lenses for excitation and the opposing one for stimulated emission.

Details

ISSN :
00222720
Volume :
180
Database :
OpenAIRE
Journal :
Journal of Microscopy
Accession number :
edsair.doi...........67d3f0ef93cc883d30a7d33a89b89249
Full Text :
https://doi.org/10.1111/j.1365-2818.1995.tb03662.x