Back to Search
Start Over
Layer-By-Layer Film Engineering for Sequential Gene Delivery
- Source :
- Nanotechnology for Nucleic Acid Delivery ISBN: 9781493990917
- Publication Year :
- 2019
- Publisher :
- Springer New York, 2019.
-
Abstract
- Layer-by-layer (LbL) films are assembled with poly(amido amine)s (PAAs), a type of polycations containing bioreducible disulfide bond, and DNA plasmids to enable LbL film degradation in physiologic conditions by reacting with glutathione or redox-active membrane proteins. The interior layer structure of the LbL films during assembly and disassembly is studied by atomic force microscopy (AFM), ellipsometry, dynamic light scattering (DLS), and fluorescence spectroscopy. Insertion of barrier layers in bioreducible LbL films is necessary to stabilize the interior layer structure and slow down the film degradation rate to achieve sequential gene delivery. Localized gene delivery from the LbL films is demonstrated using human embryonic kidney 293 (HEK 293) cells.
- Subjects :
- Materials science
HEK 293 cells
Layer by layer
02 engineering and technology
Gene delivery
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
Fluorescence spectroscopy
0104 chemical sciences
Chemical engineering
Dynamic light scattering
Ellipsometry
Degradation (geology)
0210 nano-technology
Layer (electronics)
Subjects
Details
- ISBN :
- 978-1-4939-9091-7
- ISBNs :
- 9781493990917
- Database :
- OpenAIRE
- Journal :
- Nanotechnology for Nucleic Acid Delivery ISBN: 9781493990917
- Accession number :
- edsair.doi...........6952b07318fbc3c9917dad8dedf24c10