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Isolation and characterization of a high-affinity ammonium transporter ApAMT1;1 in alligatorweed
- Source :
- Plant Growth Regulation. 89:321-330
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- In aquatic fields, ammonium (NH4+) is the most preferred nitrogen (N) source used by plants. The uptake of NH4+ is facilitated by the family of ammonium transporters (AMTs). However, the molecular functions of AMTs in aquatic plants are largely unknown. In this work, a new NH4+ transporter encoding gene, ApAMT1;1, was isolated from the typical aquatic plant alligatorweed, using degenerated primers and rapid amplification of cDNA end (RACE) techniques. Quantitative real time PCR showed that ApAMT1;1 was predominantly expressed in roots, and significantly induced by NH4+ starvation in all tested tissues, including leaves, stems and roots. Functional determination and 15N-labeled ammonium uptake assays in yeast cells indicated that ApAMT1;1 was a typical high-affinity transporter, with a 38.6 μM Km value, and the phosphorylation site T469 was required to retain its NH4+ uptake capacity. Further analyses with Met sulfoximine (MSX), a NH4+ assimilation inhibitor, demonstrated that ApAMT1;1-mediated NH4+ uptake might be feedback regulated by the internal NH4+ accumulation. Our results reveal a functional role of ApAMT1;1 in the uptake and transport of NH4+ in aquatic plants.
- Subjects :
- inorganic chemicals
0106 biological sciences
0301 basic medicine
Physiology
Chemistry
food and beverages
Plant physiology
chemistry.chemical_element
Transporter
Plant Science
01 natural sciences
Nitrogen
Yeast
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Biochemistry
Aquatic plant
Complementary DNA
Ammonium
Agronomy and Crop Science
Gene
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15735087 and 01676903
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Plant Growth Regulation
- Accession number :
- edsair.doi...........6a1d391a01bb898655ffcae4850c1cc6