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Cycling Temperature Strategy: A Method to Improve the Efficiency of Crystallization Condition Screening of Proteins
- Source :
- Crystal Growth & Design. 8:4227-4232
- Publication Year :
- 2008
- Publisher :
- American Chemical Society (ACS), 2008.
-
Abstract
- Temperature is generally considered as an important factor in protein crystallization. Such is true because crystals usually grow at a preferable temperature in a certain crystallization solution. If a nonsuitable temperature is used, the solution will not yield crystals. However, it is difficult to decide the best temperature suited for screening the crystallization condition of proteins. In this study, it was found out that, compared to constant temperature, a variation in a reasonable range can result in a more efficient crystallization screening. Using the Sparse Matrix Screen with the screening kit Index, this study tested nine commercially available proteins and proved that, compared to the conventional constant temperature strategy, a varying temperature strategy can actually increase the possibility of obtaining crystals. Consequently, the cycling temperature strategy (CTS) is then proposed to be utilized in most screening tasks when the suitable crystallization temperature is unknown.
Details
- ISSN :
- 15287505 and 15287483
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Crystal Growth & Design
- Accession number :
- edsair.doi...........6b0083a790ec0e39410768c9efab7e5d