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Determination of isovitexin from Lespedeza cuneata using a validated HPLC-UV method

Authors :
Jajung Ku
Seong Jae Lee
Leo Adrianne Paje
Sang-Woo Yoo
Ju Sung Lee
Sanghyun Lee
Source :
Journal of Applied Biological Chemistry. 64:63-67
Publication Year :
2021
Publisher :
Korean Society for Applied Biological Chemistry, 2021.

Abstract

Isovitexin, a marker compound with various pharmacological activities, in Lespedeza cuneata, was analyzed using high performance liquid chromatography coupled with UV (HPLC/UV). There are no previous reports on using L. cuneata as the source material for the quantification of isovitexin. In this study, we developed an optimized method using HPLC-UV analysis, which was validated using various parameters. Our method demonstrated high specificity, and good separation of the chromatographic peak was achieved. Parameters such as linearity (r2>0.9997), precision, and accuracy indicated that our proposed analytical method had good reliability and sensitivity. These results demonstrate the utility and convenience of our method for rapidly quantifying isovitexin in L. cuneata extracts.

Details

ISSN :
22347941 and 19760442
Volume :
64
Database :
OpenAIRE
Journal :
Journal of Applied Biological Chemistry
Accession number :
edsair.doi...........6c7d34f8a154da8f4c6300792231cf4e
Full Text :
https://doi.org/10.3839/jabc.2021.010