Back to Search Start Over

Antihypertensive effect of methanol leaf extract of Azadirachta indica is mediated through suppression of renal caspase 3 expressions on Nω-Nitro-l-arginine methyl ester induced hypertension

Authors :
Idayat Titilayo Gbadamos
Oluwafemi Omoniyi Oguntibeju
Adebowale Bernard Saba
Temidayo Olutayo Omobowale
Adeolu Alex Adedapo
Ebunoluwa Racheal Asenuga
Anofi Ashafa
Blessing Seun Ogunpolu
Momoh A. Yakubu
Olumuyiwa Abiola Adejumobi
Olufunke Olubunmi Falayi
Temitayo Olabisi Ajibade
Olufunke Eunice Ola-Davies
Jeremiah Moyinoluwa Afolabi
Fisayo Olutayo Ugbor
Ademola Adetokunbo Oyagbemi
Source :
Pharmacognosy Research. 12:460
Publication Year :
2020
Publisher :
EManuscript Technologies, 2020.

Abstract

Background: Azadirachta indica (AI) Adr Juss (Meliaceae), known as neem, has been used traditionally for the treatment of various disease conditions including obesity and hypertension. Objective: The antihypertensive effect and mechanism of action of modulatory effect of AI were investigated after the induction of hypertension using Nω-nitro-L-arginine methyl ester (L-NAME). Materials and Methods: Five groups of ten rats divided as follows: Control; L-NAME (40 mg/kg); L-NAME + 100 mg/kg AI; L-NAME and 200 mg/kg ?AI; and L-NAME and Enalapril (25 mg/kg) were used. Results: following the application of L-NAME, hypertension (elevated systolic, diastolic, mean arterial blood pressures) and increased levels of oxidative stress markers were observed in rats. Immunohistochemistry showed increased caspase-3 expressions in hypertensive rats compared to normotensive rats. Conversely, AI treatment resulted in restoration of physiological antioxidant status and normotension, comparable to the standard antihypertensive agent enalapril. Conclusion: AI leaf is a good candidate for the management of high blood pressure.

Details

ISSN :
09748490
Volume :
12
Database :
OpenAIRE
Journal :
Pharmacognosy Research
Accession number :
edsair.doi...........6d901252fe72d5feefc5d805cbd2fbc0
Full Text :
https://doi.org/10.4103/pr.pr_10_20