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Elucidate Genetic Diversity and Population Structure of Bread Wheat (Triticum Aestivum L.) Cultivars Using IRAP and REMAP Markers
- Source :
- Journal of Crop Science and Biotechnology. 22:139-151
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Analysis of genetic diversity and population structure in bread wheat is an essential step in their conservation, utilization, and breeding. Retrotransposons are ubiquitous and abundant a throughout the plant genomes, therefore extensively used as ideal molecular markers for genetic variability, DNA fingerprinting and genetic mapping studies in plant species. In the current research, we used two retrotransposon-based marker systems, inter-retrotransposon amplified polymorphisms (IRAPs), and the retrotransposon-microsatellite amplified polymorphisms (REMAPs) markers to evaluate the genetic diversity and survey activity of long terminal repeat retrotransposon (LTR-retrotransposon) elements in a collection of 49 bread wheat (Triticum aestivum L.) cultivars that mainly bred in Iran. In general, 90 and 126 loci were amplified using 9 IRAP and 20 REMAP primers, respectively. Both techniques produced a satisfactory number of bands for cultivar analysis; however, the technique IRAP, particularly single primer Nikita generated a large number of bands, indicating the wide activity of Nikita family under various environmental conditions of bread wheat. The percentage of polymorphic loci (PPL) in the studied collection for IRAP and REMAP markers was 81.78 and 86.40%, respectively. A model-based Bayesian method, Principal coordinate analysis (PCoA) and cluster analysis using Minimum Evolution (ME) algorithm hinted of the existence of two groups. This grouping was in agreement with the growing season and conformed by the high within-group bootstrap value. These results demonstrated that these markers developed using transpositionally active retrotransposons (RTNs) are efficient and reliable markers in determining level of genetic diversity and population structure in bread wheat in breeding programs.
- Subjects :
- 0106 biological sciences
Genetics
Genetic diversity
food and beverages
Retrotransposon
04 agricultural and veterinary sciences
Plant Science
Biology
01 natural sciences
Long terminal repeat
Gene mapping
DNA profiling
040103 agronomy & agriculture
0401 agriculture, forestry, and fisheries
Cultivar
Genetic variability
Primer (molecular biology)
Agronomy and Crop Science
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 20058276 and 19759479
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Journal of Crop Science and Biotechnology
- Accession number :
- edsair.doi...........6da0c59e49b1f951e59537e966ca2cd8