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Assessment of Microbial Diversity in Saudi Springs by Culture-Dependent and Culture-Independent Methods
- Source :
- Geomicrobiology Journal. :1-11
- Publication Year :
- 2016
- Publisher :
- Informa UK Limited, 2016.
-
Abstract
- Culture-dependent and culture-independent methods were used to evaluate the microbial diversity in two hot springs of the Aljouf region in Saudi Arabia, including Qasr Kaff and Ain Hawas. Physicochemical characteristics of the springs were examined to establish their effect on the biodiversity of thermophilic bacteria and fungi. We employed culture-dependent techniques to study microbial diversity using four different complex media for bacteria and fungi. In addition, the direct count for algal populations from two springs was investigated. We surveyed the microbial diversity in water and sediment samples from both springs by denaturing gradient gel electrophoresis (DGGE) and clone library construction. Bacillariophycaea (18 species) was the most diverse group, followed by Cyanophyceaea. Bacterial isolates closer to the genera Bacillus spp., Geobacillus, Thermoactinomyces, and unidentified actinobacteria were recovered. Fungal isolates were related to Aspergillus, Pezizaceae, Penicillium, Acremoni...
- Subjects :
- 0301 basic medicine
biology
Library
Ecology
Thermophile
Biodiversity
biology.organism_classification
Microbiology
Actinobacteria
03 medical and health sciences
030104 developmental biology
Pezizaceae
Penicillium
Botany
Earth and Planetary Sciences (miscellaneous)
Environmental Chemistry
human activities
Temperature gradient gel electrophoresis
Bacteria
General Environmental Science
Subjects
Details
- ISSN :
- 15210529 and 01490451
- Database :
- OpenAIRE
- Journal :
- Geomicrobiology Journal
- Accession number :
- edsair.doi...........6ed41cebc23149784748e9738b2ed8e5