Racemisation studies of a novel coupling reagent for solid phase peptide synthesis

A novel coupling reagent ethyl 1-hydroxy-1H-1,2,3-triazole-4-carboxylate has been developed for use in conjunction with diisopropylcarbodiimide for solid phase peptide synthesis. The synthesis and application of this reagent in solid phase peptide synthesis has been reported. Here we report a systematic study into racemisation upon activation and coupling of single amino acids and dipeptides with this reagent. A novel coupling reagent ethyl 1-hydroxy-1H-1,2,3-triazole-4-carboxylate has been developed for use in conjunction with diisopropylcarbodiimide for solid phase peptide synthesis. The synthesis and application of this reagent in solid phase peptide synthesis has been reported. Here we report a systematic study into racemisation upon activation and coupling of single amino acids and dipeptides with this reagent.