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Zebrafish Tumor Graft Transplantation to Grow Tumors In Vivo That Engraft Poorly as Single Cell Suspensions

Authors :
Myron S. Ignatius
Kunal Baxi
Amanda Lipsitt
Anil K. Challa
Nicole R. Hensch
Jiangfei Chen
Rodrigo Moreno-Campos
Eleanor Y. Chen
Source :
Zebrafish. 18:293-296
Publication Year :
2021
Publisher :
Mary Ann Liebert Inc, 2021.

Abstract

Angiosarcoma is a clinically aggressive tumor with a high rate of mortality. It can arise in vascular or lymphatic tissues, involve any part of the body, and aggressively spread locally or metastasize. Angiosarcomas spontaneously develop in the tp53 deleted (tp53del/del) zebrafish mutant. However, established protocols for tumor dissection and transplantation of single cell suspensions of angiosarcoma tumors result in inferior implantation rates. To resolve these complications, we developed a new tumor grafting technique for engraftment of angiosarcoma and similar tumors in zebrafish, which maintains the tumor microenvironment and has superior rates of engraftment.

Details

ISSN :
15578542 and 15458547
Volume :
18
Database :
OpenAIRE
Journal :
Zebrafish
Accession number :
edsair.doi...........70485ab97281fc2565bf001f73ce68a1
Full Text :
https://doi.org/10.1089/zeb.2021.0006