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Screening for theFIG-ROS1fusion in biliary tract carcinomas by nested PCR
- Source :
- Genes, Chromosomes and Cancer. 53:1033-1040
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- ROS1 rearrangements have been detected in a variety of tumors and are considered as suitable targets of anticancer therapies. We developed a new, quick, specific, and sensitive PCR test to screen for the FIG-ROS1 fusion and applied it to a series of Italian patients with bile duct carcinoma (BTC). Formalin-fixed, paraffin-embedded tissues, derived from 65 Italian BTC patients, and six cell lines were analyzed by nested PCR to investigate the prevalence of a previously reported FIG-ROS1 fusion. The specificity and sensitivity of nested PCR were investigated in FIG-ROS1 positive U118MG cells in reconstitution experiments with peripheral blood mononuclear cells. We found that six out of 65 (9%) BTC patients were positive for the FIG-ROS1 fusion, comprising two out of 14 (14%) gallbladder carcinoma (GBC) patients and four out of 25 (16%) extrahepatic cholangiocarcinoma (ECC) patients. None of the 26 intrahepatic cholangiocarcinoma cases harbored the FIG-ROS1 fusion. All the cell lines were negative for this variant. In conclusion, 14-16% of GBC and ECC were positive for FIG-ROS1. This may have clinical implications, since these patients will potentially benefit from the treatment with specific ROS1 inhibitors.
- Subjects :
- Cancer Research
medicine.medical_specialty
Gallbladder
Bile Duct Neoplasm
Biology
Bile Duct Carcinoma
medicine.disease
Gastroenterology
medicine.anatomical_structure
Biliary tract
Internal medicine
Immunology
Genetics
medicine
ROS1
Carcinoma
Nested polymerase chain reaction
Intrahepatic Cholangiocarcinoma
Subjects
Details
- ISSN :
- 10452257
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Genes, Chromosomes and Cancer
- Accession number :
- edsair.doi...........70a38020a6cd7c8d1b795c54f4646e26