Antibacterial resistance Escherichia coli and Aeromonas hydrophila on alkaloid from marine sponge Callyspongia sp

Research on the bioactivity of a compound from Callyspongia sp. as an antibacterial agent against Escherichia coli and Aeromonas hydrophila resistants was conducted. The active metabolite was isolated based on bioassay-guided separation with several steps of chromatography. The FTIR data showed the vibration at 1637.4 cm−1 as C-N imine functional group with the fingerprint region at 1449.0 cm−1 indicated the presence of alkaloid as the active compound. The results of this study informed the inhibitory zone diameter of the extract against E. coli showed an area as wide as 8.10 mm at the concentration of 80 μg mL−1 compared to the inhibitory zone diameter of the positive control Chloramphenicol as wide as 7.50 mm at the concentration of 40 μg mL−1. However, the inhibitory zone of the extract against A. hydrophila displayed an area 8.50 mm in diameter at the concentration of 20 μg mL−1 compared to the zone diameter of the positive control Chloramphenicol as wide as 7.25 mm at the concentration of 40 μg mL−1. Based on the bioassay results, the alkaloid compound from Callyspongia sp. has potential activity against the Gram-positive bacterial resistant of Escheria coli and the Gram-negative bacterial resistant of Aeromonas hydrophila.