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Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity
- Source :
- BioChip Journal. 15:195-203
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration of anti-spike protein (SP) IgG antibodies using magnetic beads with immobilized human SARS-CoV SP. The enhanced specificity of the purified antibodies to the NP of SARS-CoV-2 was demonstrated using an immunoassay with anti-NP IgG antibodies after the isolation and filtration steps. The binding constants (Kd) of the purified anti-NP IgG antibodies to the NP of SARS-CoV-2 and the SP of SARS-CoV were estimated using a surface plasmon resonance biosensor (SPR). A competitive assay using the two-step purified anti-NP IgG antibodies from pig serum demonstrated (a) the detection of SARS-CoV-2 in viral fluid and (b) the discrimination of SARS-CoV-2 from SARS-CoV, MERS-CoV, and CoV strain 229E in viral fluids.
- Subjects :
- 2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19)
viruses
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Biomedical Engineering
Bioengineering
Surface plasmon resonance biosensor
02 engineering and technology
01 natural sciences
medicine
Electrical and Electronic Engineering
skin and connective tissue diseases
biology
Strain (chemistry)
medicine.diagnostic_test
Chemistry
fungi
010401 analytical chemistry
virus diseases
021001 nanoscience & nanotechnology
Molecular biology
respiratory tract diseases
0104 chemical sciences
Nucleoprotein
Immunoassay
biology.protein
Antibody
0210 nano-technology
Biotechnology
Subjects
Details
- ISSN :
- 20927843 and 19760280
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- BioChip Journal
- Accession number :
- edsair.doi...........72c383685ede48d1c9a10647175609c4