An improved synthesis of N-acetoxy-N-acetyl-2-aminofluorene-9-14C using an extractive acylation technique

A rapid, high yield (90–100%) method for the preparation of N-acetoxy-N-acetyl-2-aminofluorene-9-14C (N-AcO-AAF-9-14C) from N-hydroxy-N-acetyl-2-aminofluorene-9-14C (N-OH-AAF-9-14C) is described. The procedure consists of the in situ formation of a quaternary ammonium salt of N-OH-AAF-9-14C in a two-phase system and subsequent acetylation of this salt with acetic anhydride. The specific activity of the product was 40.6 mCi/mmole.