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Protective effect of 2,2,2-trichloroethanol on peptidoglycan-induced inflammation in murine macrophages
- Source :
- African Journal of Pharmacy and Pharmacology. 8:256-262
- Publication Year :
- 2014
- Publisher :
- Academic Journals, 2014.
-
Abstract
- The aim of this study was to investigate the effect of 2,2,2-trichloroethanol on peptidoglycan-induced murine macrophages inflammation. The effect of 2,2,2-trichloroethanol on the production of TNF-α and IL-6 by murine peritoneal macrophages with peptidoglycan-stimulation were investigated. Also, RAW264.7 macrophages transfected with a NF-κB luciferase reporter plasmid with the stimulation of peptidoglycan were used to test the effect of 2,2,2-trichloroethanol on NF-κB activity. Flow cytometry and Western blotting were used to check the expression levels of toll-like receptor 2 on treated RAW264.7 macrophages. 2,2,2-Trichloroethanol decreased the unregulated levels of IL-6 and TNF-α produced by the peritoneal macrophages stimulation with peptidoglycan. The NF-κB activities of the RAW264.7 macrophages stimulated by peptidoglycan were decreased. 2,2,2-Trichloroethanol decreased the peptidoglycan-induced murine macrophage inflammation response. Key words: 2,2,2-Trichloroethanol, peptidoglycan, inflammation, macrophage.
Details
- ISSN :
- 19960816
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- African Journal of Pharmacy and Pharmacology
- Accession number :
- edsair.doi...........767ff806f2c84d98bdce0ffcc641ed4f