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Effect of low-frequency ultrasound on double-strand breaks in giant DNA molecules

Authors :
Yukihiro Kagawa
Kenichi Yoshikawa
Hiraku Tabata
Takahiro Kenmotsu
Naoki Ogawa
Yuko Yoshikawa
Yoshiaki Watanabe
Kenji Yoshida
Source :
Applied Physics Letters. 103:063705
Publication Year :
2013
Publisher :
AIP Publishing, 2013.

Abstract

Double-strand breaks in giant DNA molecules caused by continuous ultrasound at a frequency of 30 kHz were quantified using single-molecule observations. The effect of the sound pressure was investigated by placing a tube containing DNA solution under an anti-node of the acoustic standing wave. Almost no breaks occurred below the threshold sound pressure. Above this threshold, the probability of strand breaks increased linearly with sound pressure. Acoustic cavitation detected with a hydrophone strongly suggests that the main mechanism of the DNA strand break is via cavitation generated by the ultrasound.

Details

ISSN :
10773118 and 00036951
Volume :
103
Database :
OpenAIRE
Journal :
Applied Physics Letters
Accession number :
edsair.doi...........76db90a93072da8bd367894ea2d4fe5e
Full Text :
https://doi.org/10.1063/1.4818125