Relationships Between Infectious Virus, Hemagglutinin and Complement-Fixing Antigen of ECHO Virus, Type 19

SummaryThe CF antigen of ECHO 19 virus can be separated from the hemagglutinin and infectious virus particle. Although the 3 properties, based on sedimentation, indicated a close association, nevertheless, during virus multiplication each emerged at different periods. Infectivity and HA properties were labile to heat and UV irradiation; the CF was stable to both heat and UV. Absorption of infected TC fluid with human “O”erythrocytes removed more than 99% of infectious virus and hemagglutinin. The CF antigen was unaffected by absorption which indicated that the infectivity and HA were more closely combined than the CF. The available data suggested that the CF antigen is an integral part of the virus particle (non-infectious).