Back to Search
Start Over
The effects of <scp>11‐ketotestosterone</scp> implants on transcript levels of gonadotropin receptors, and foxl2 and dmrt1 genes in the Previtellogenic ovary of cultured beluga ( <scp> Huso huso </scp> )
- Source :
- Journal of Fish Biology. 97:374-382
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- The in vivo effect of 11-ketotestosterone (11KT) on transcript levels of the gonadotropin receptors (fshr and lhr) and sex differentiation-related genes (dmrt1 and foxl2) was examined in the ovaries of immature female beluga. For this purpose, six fish were treated with implants containing 2.5 mg 11KT and a placebo group of six females of the same age and gametogenic stage were given a blank implant. The implants were intraperitoneally inserted into 4-year-old females at the previtellogenic stage (mean body weight 5580 ± 165 g) and maintained under culture conditions for 8 weeks. Ovary samples for gene expression analysis of lhr, fshr, dmrt1 and foxl2 were collected by biopsy at 3 and 8 weeks post implantation. Diameters of oocytes increased in response to 11KT treatment, both at 3 and at 8 weeks post implantation, but no obvious changes were evident in cytology. Three weeks of 11KT treatment did not affect target gene expression, but a tendency for a time-dependent decrease of lhr and dmrt1 mRNA levels was observed in both treatment and placebo groups. By 8 weeks of treatment, however, 11KT implants provoked the upregulation of fshr and foxl2 transcript levels. Furthermore, lhr and dmrt1 transcript abundances recovered by 8 weeks of exposure in both blank- and 11KT-implanted beluga. These results suggest that 11KT, either directly or indirectly, may affect gametogenesis and regulate some key components of the reproductive axis in female beluga.
- Subjects :
- 0106 biological sciences
biology
medicine.drug_class
010604 marine biology & hydrobiology
Beluga
Huso
Ovary
Aquatic Science
biology.organism_classification
010603 evolutionary biology
01 natural sciences
Andrology
chemistry.chemical_compound
medicine.anatomical_structure
chemistry
medicine
11-Ketotestosterone
Implant
Gonadotropin
Receptor
Ecology, Evolution, Behavior and Systematics
Gametogenesis
Subjects
Details
- ISSN :
- 10958649 and 00221112
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Journal of Fish Biology
- Accession number :
- edsair.doi...........787bebabe0a1d65c159167d43d3086f9