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99m TcâCeftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis
- Source :
- Journal of Medical Imaging and Radiation Oncology. 63:61-68
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Introduction The diagnosis of diabetic foot osteomyelitis is crucial and challenging for the proper management of diabetic foot. 99m Tc labelled Ceftizoxime was used as a non-invasive diagnostic agent for diabetic foot osteomyelitis. Methods Ceftizoxime [CFT], a third generation cephalosporin, was used in a simple and direct method for the synthesis of 99m TcO4 - labelled infection imaging agent with stannous chloride as reducing agent. Its radiochemical purity was checked by thin Layer chromatography. Partition co-efficient was measured with phosphate buffer and chloroform. The radiochemical complex was injected to control and infected animal model for 3 hours in-vivo localization studied with the help of dual head gamma camera. The labelled complexes were injected to 5 patients of known type II diabetes mellitus suspected of diabetic foot osteomyelitis. All patients underwent dynamic and static 99m Tc-MDP and 99m Tc-CFT scans. Results The synthesized radio labelled complex was 98.8% pure, with hydrophilic character. When injected to animal model, at 120 minutes, 49.3% was localized in foci of infection with 3.35% in liver and excretion through kidney. Human studies were interpreted as true or false positive and true or false negative based on bone histopathology/culture and clinical follow-up. We found that of 5 patients, 2 were true positive, 2 as true negative with no false positive or negative and 01 patient had soft tissue infection. Conclusion This study showed that 99m Tc-CFT labelled complex could be used for detection of diabetic foot osteomyelitis; however, further confirmation of results with a larger patient population would be optimal.
- Subjects :
- medicine.medical_specialty
Kidney
Human studies
business.industry
medicine.drug_class
Osteomyelitis
Cephalosporin
Urology
medicine.disease
Diabetic foot
030218 nuclear medicine & medical imaging
Excretion
03 medical and health sciences
0302 clinical medicine
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Ceftizoxime
medicine
Radiology, Nuclear Medicine and imaging
Histopathology
business
medicine.drug
Subjects
Details
- ISSN :
- 17549485 and 17549477
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Imaging and Radiation Oncology
- Accession number :
- edsair.doi...........790b91ad50e0842c764003c3a6c3913a
- Full Text :
- https://doi.org/10.1111/1754-9485.12841