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Cyclodextrin and its derivatives enhance protein crystallization by grafted on crystallization plates
- Source :
- Journal of Crystal Growth. 536:125591
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Protein drugs are attracting increasing attention. Eight of best-selling drugs in worldwide are proteins. Crystallization is an important protein purification method; however, it remains a bottleneck step. Heterogeneous nucleate is an effective method of enhancing protein crystallization. Cyclodextrin has been widely used in pharmaceutics, and our previous study showed that it is an effective heterogeneous nucleate for enhancing protein crystallization, and it may be widely used in purification of protein drugs. However, cyclodextrin must be added into the crystallization plate wells by hand, which is labor intensive and is not beneficial to automated crystallization screening. We grafted β-cyclodextrin (β-CD) and its derivatives (i.e., p-toluenesulfonyl-β-cyclodextrin [PTCD], mono-(6-(1,6-hexamethylenediamine)-6-deoxy)-β-cyclodextrin [MHCD] and mercapto-β-cyclodextrin [MCD]) on crystallization plates and tested their effects on protein crystallization. Protein crystallization success rate was improved, particularly for the PTCD-grafted group. This is an easy method to facilitate protein crystallization and can be widely applied for automatic crystallization screening.
- Subjects :
- 010302 applied physics
chemistry.chemical_classification
Cyclodextrin
Chemistry
Nucleation
02 engineering and technology
021001 nanoscience & nanotechnology
Condensed Matter Physics
01 natural sciences
Combinatorial chemistry
law.invention
Inorganic Chemistry
law
0103 physical sciences
Protein purification
Materials Chemistry
Pharmaceutics
Crystallization
0210 nano-technology
Protein crystallization
Subjects
Details
- ISSN :
- 00220248
- Volume :
- 536
- Database :
- OpenAIRE
- Journal :
- Journal of Crystal Growth
- Accession number :
- edsair.doi...........7a87eb6fd53d8067c8ac08db1a123029