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The Challenges of Interpreting Phosphoproteomics Data: A Critical View Through the Bioinformatics Lens
- Source :
- Computational Intelligence Methods for Bioinformatics and Biostatistics ISBN: 9783319443317, CIBB
- Publication Year :
- 2016
- Publisher :
- Springer International Publishing, 2016.
-
Abstract
- During the last decade, there has been great progress in high-throughput (HTP) phosphoproteomics and hundreds or even thousands of phosphorylation sites (p-sites) can now be detected in a single experiment. This success is attributable to a combination of very sensitive Mass Spectrometry instruments, better phosphopeptide enrichment techniques and bioinformatics software that are capable of detecting peptides and localizing p-sites. These new technologies have opened up a whole new level of gene regulation to be studied, with great potential for therapeutics and synthetic biology. Nevertheless, many challenges remain to be resolved; these concern the biases and noise of these proteomic technologies, the biological noise that is present, as well as the incompleteness of the current datasets. Despite these problems, the datasets published so far appear to represent a good sample of a complete phosphoproteome of some organisms and are capable of revealing their major properties.
- Subjects :
- 0301 basic medicine
Phosphorylation sites
Emerging technologies
Computer science
Phosphopeptide
Phosphoproteomics
Bioinformatics
Mass spectrometry
03 medical and health sciences
Synthetic biology
030104 developmental biology
0302 clinical medicine
030220 oncology & carcinogenesis
Phosphorylation
Subjects
Details
- ISBN :
- 978-3-319-44331-7
- ISBNs :
- 9783319443317
- Database :
- OpenAIRE
- Journal :
- Computational Intelligence Methods for Bioinformatics and Biostatistics ISBN: 9783319443317, CIBB
- Accession number :
- edsair.doi...........7c8f299e1885f9a9ad1263a71ec0f7d0
- Full Text :
- https://doi.org/10.1007/978-3-319-44332-4_15