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Einstaz der Fermenter-Dialyse-Kultur zur Züchtung kapseltragender Bakterien mit klinischer Bedeutung I.Escherichia coli K1
- Source :
- Journal of Basic Microbiology. 27:329-339
- Publication Year :
- 1987
- Publisher :
- Wiley, 1987.
-
Abstract
- For mass culture of Escherichia coli K1 a fermenter dialysis culture technique based on an artificial hollowfiber kidney and substrate feeding is described and compared with conventional shaking culture and fermenter batch culture techniques. Important growth parameters as bacterial dry weight, optical density and live cell counts demonstrate the advantage of this new method. On the basis of bacterial yields, effectivity had increased about 100 fold. The synthesis of K1 capsular polysaccharides, which is influenced by a number of factors, proved to be good. Thus it was possible to obtain the quantities of native capsular substances necessary for bed-side diagnostics and the investigation of this virulence marker.
- Subjects :
- chemistry.chemical_classification
Mass culture
biology
Virulence
Industrial fermentation
General Medicine
biology.organism_classification
medicine.disease_cause
Polysaccharide
Applied Microbiology and Biotechnology
Enterobacteriaceae
Microbiology
chemistry
medicine
Pathogen
Escherichia coli
Bacteria
Subjects
Details
- ISSN :
- 15214028 and 0233111X
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Journal of Basic Microbiology
- Accession number :
- edsair.doi...........7cb16ec492f280fe6e9a560f42b052dc
- Full Text :
- https://doi.org/10.1002/jobm.3620270611