Back to Search
Start Over
Enhanced production of seed oil with improved fatty acid composition by overexpressing NAD + ‐dependent glycerol‐3‐phosphate dehydrogenase in soybean
- Source :
- Journal of Integrative Plant Biology. 63:1036-1053
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- There is growing interest in expanding the production of soybean oils (mainly triacylglycerol, or TAG) to meet rising feed demand and address global energy concerns. We report that a plastid-localized glycerol-3-phosphate dehydrogenase (GPDH), encoded by GmGPDHp1 gene, catalyzes the formation of glycerol-3-phosphate (G3P), an obligate substrate required for TAG biosynthesis. Overexpression of GmGPDHp1 increases soybean seed oil content with high levels of unsaturated fatty acids (FAs), especially oleic acid (C18:1), without detectably affecting growth or seed protein content or seed weight. Based on the lipidomic analyses, we found that the increase in G3P content led to an elevated diacylglycerol (DAG) pool, in which the Kennedy pathway-derived DAG was mostly increased, followed by PC-derived DAG, thereby promoting the synthesis of TAG containing relatively high proportion of C18:1. The increased G3P levels induced several transcriptional alterations of genes involved in the glycerolipid pathways. In particular, genes encoding the enzymes responsible for de novo glycerolipid synthesis were largely upregulated in the transgenic lines, in-line with the identified biochemical phenotype. These results reveal a key role for GmGPDHp1-mediated G3P metabolism in enhancing TAG synthesis and demonstrate a strategy to modify the FA compositions of soybean oils for improved nutrition and biofuel.
- Subjects :
- 0106 biological sciences
0301 basic medicine
chemistry.chemical_classification
food and beverages
Dehydrogenase
Plant Science
Metabolism
01 natural sciences
Biochemistry
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Oleic acid
chemistry.chemical_compound
030104 developmental biology
Enzyme
Glycerol-3-phosphate dehydrogenase
chemistry
Biosynthesis
Lipidomics
lipids (amino acids, peptides, and proteins)
010606 plant biology & botany
Diacylglycerol kinase
Subjects
Details
- ISSN :
- 17447909 and 16729072
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Plant Biology
- Accession number :
- edsair.doi...........7d3eb46fde8e18bb7c55789db6605c5d