Back to Search
Start Over
Characterization of an Intermediate Filament Protein from the Platyhelminth,Dugesia japonica
- Source :
- Protein & Peptide Letters. 27:432-446
- Publication Year :
- 2020
- Publisher :
- Bentham Science Publishers Ltd., 2020.
-
Abstract
- Background:Intermediate Filaments (IFs) are major constituents of the cytoskeletal systems in animal cells.Objective:To gain insights into the structure-function relationship of invertebrate cytoplasmic IF proteins, we characterized an IF protein from the platyhelminth, Dugesia japonica, termed Dif-1.Method:cDNA cloning, in situ hybridization, immunohistochemical analysis, and IF assembly experiments in vitro using recombinant Dif-1, were performed for protein characterization.Results:The structure deduced from the cDNA sequence showed that Djf-1 comprises 568 amino acids and has a tripartite domain structure (N-terminal head, central rod, and C-terminal tail) that is characteristic of IF proteins. Similar to nuclear IF lamins, Djf-1 contains an extra 42 residues in the coil 1b subdomain of the rod domain that is absent from vertebrate cytoplasmic IF proteins and a nuclear lamin-homology segment of approximately 105 residues in the tail domain; however, it contains no nuclear localization signal. In situ hybridization analysis showed that Djf-1 mRNA is specifically expressed in cells located within the marginal region encircling the worm body. Immunohistochemical analysis showed that Djf-1 protein forms cytoplasmic IFs located close to the microvilli of the cells. In vitro IF assembly experiments using recombinant proteins showed that Djf-1 alone polymerizes into IFs. Deletion of the extra 42 residues in the coil 1b subdomain resulted in the failure of IF formation.Conclusions:Together with data from other histological studies, our results suggest that Djf- 1 is expressed specifically in anchor cells within the glandular adhesive organs of the worm and that Djf-1 IFs may play a role in protecting the cells from mechanical stress.
- Subjects :
- 0303 health sciences
biology
Chemistry
General Medicine
biology.organism_classification
Biochemistry
Cell biology
03 medical and health sciences
0302 clinical medicine
Structural Biology
Cytoplasm
Complementary DNA
Intermediate Filament Protein
Dugesia japonica
Cytoskeleton
Intermediate filament
030217 neurology & neurosurgery
Nuclear localization sequence
Lamin
030304 developmental biology
Subjects
Details
- ISSN :
- 09298665
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Protein & Peptide Letters
- Accession number :
- edsair.doi...........7df0439cc446528e189ee9bdd4fca4ec
- Full Text :
- https://doi.org/10.2174/0929866526666191025102902