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Isolation and quantitative analysis of hydroxylysine glycosides
- Source :
- Chromatographia. 28:497-501
- Publication Year :
- 1989
- Publisher :
- Springer Science and Business Media LLC, 1989.
-
Abstract
- Methods are described for the isolation of galactosyl hydroxylysine and glucosylgalactosyl hydroxylysine from collagen and for the quantitative analysis of these hydroxylysine glycosides. The isolation procedure, based upon gel filtration and preparative ionpaired reversed-phase high-performance liquid chromatography, is simple and rapid in comparison with existing methods, allowing the production of tens of milligrams of each glycoside in a single day. Quantitative analysis of the hydroxylysine glycosides is effected by reversed-phase high-performance liquid chromatography with precolumn derivatization (usingo-phthal-dialdehyde) and fluorescence detection.
- Subjects :
- chemistry.chemical_classification
Chromatography
Organic Chemistry
Clinical Biochemistry
Size-exclusion chromatography
Glycoside
Reversed-phase chromatography
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
Hydroxylysine
chemistry.chemical_compound
chemistry
Derivatization
Quantitative analysis (chemistry)
Precolumn derivatization
Subjects
Details
- ISSN :
- 16121112 and 00095893
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Chromatographia
- Accession number :
- edsair.doi...........7e9820990c98e339ef8fcdb172bb7ce6