Back to Search
Start Over
Laser raman and infrared spectrum analysis on low-density lipoproteins purified from hen egg yolk
- Source :
- Scientific Research and Essays. 7
- Publication Year :
- 2012
- Publisher :
- Academic Journals, 2012.
-
Abstract
- Diversified proteins were separated from hen egg yolk by ammonium sulphate rapid fractionation, and pure low-density lipoprotein (LDL) was obtained after filtrating through Sephadex G-200 chromatography. After the qualitative detection ofSodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), we discovered that LDL consists of five major apoproteins. The results show that: (1) the CH2 presents asymmetric stretching and symmetric stretching mode, (2) thederivative of cholesterol C=C stretching vibration is at 1652.07 cm-1 in the Raman spectrum, and (3) a-helix bands in apolipoprotein is at 1655.12 cm-1 in the infrared spectrum. The results also suggest that laser raman and infrared spectrum analysis on LDL can provide useful information for studying the LDL structure. Key words: Low-density lipoprotein (LDL), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), C-H stretching vibration, infrared spectrum, laser raman spectrum.
- Subjects :
- Chromatography
food.ingredient
Infrared
Chemistry
Cholesterol
General Engineering
General Physics and Astronomy
General Medicine
Fractionation
General Biochemistry, Genetics and Molecular Biology
symbols.namesake
chemistry.chemical_compound
food
Biochemistry
Sephadex
Yolk
symbols
lipids (amino acids, peptides, and proteins)
General Agricultural and Biological Sciences
Raman spectroscopy
Polyacrylamide gel electrophoresis
Lipoprotein
Subjects
Details
- ISSN :
- 19922248
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Research and Essays
- Accession number :
- edsair.doi...........810e0f395d9cf8b0eaaec863f3f851c3