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Amperometric biosensor for bisphenol A based on a glassy carbon electrode modified with a nanocomposite made from polylysine, single walled carbon nanotubes and tyrosinase
- Source :
- Microchimica Acta. 180:989-996
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- We have prepared a nanocomposite consisting of single-walled carbon nanotubes and polylysine. It was characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, and by UV/vis and FTIR spectroscopy. Tyrosinase was covalently immobilized on the nanocomposite, and the resulting bioconjugate deposited on a glassy carbon electrode to form a biosensor for bisphenol A. The biosensor was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. Under optimized experimental conditions, the biosensor gives a linear response to bisphenol A in the 4.00 nM to 11.5 μM concentration range. Its sensitivity is 788 mA M−1 cm−2, and the lower detection limit is 0.97 nM (at an S/N of 3). The biosensor shows good repeatability, reproducibility and long-term stability. In a preliminary practical application, it was successfully applied to the determination of bisphenol A in leachates of plastic spoons.
Details
- ISSN :
- 14365073 and 00263672
- Volume :
- 180
- Database :
- OpenAIRE
- Journal :
- Microchimica Acta
- Accession number :
- edsair.doi...........81cde204c2d831b606444bbaa16066b8