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Development of microsatellite DNA loci from the wood stork (Aves, Ciconiidae, Mycteria americana)

Authors :
Silvia Nassif Del Lama
A. Lawrence Bryan
Alessandra M. Tomasulo‐Seccomandi
Travis C. Glenn
Nancy A. Schable
I. Lehr Brisbin
Source :
Molecular Ecology Notes. 3:563-566
Publication Year :
2003
Publisher :
Wiley, 2003.

Abstract

We isolated 11 polymorphic microsatellite loci for wood stork ( Mycteria americana ). Polymerase chain reaction (PCR) primers and conditions are described for the amplification of five dinucleotide, one trinucleotide and five tetranucleotide microsatellite loci. The PCR primers were tested on two wood stork populations, Fazenda Ipiranga, Mato Grosso, Brazil ( n = 11) and Tamiami West, Everglades, Florida, USA ( n = 20). The primers yielded two to four alleles per locus, an observed heterozygosity of 0.0–0.727 and a polymorphic information content of 0.048–0.604. The low level of polymorphism for these markers is consistent with previous studies of this species.

Details

ISSN :
14718286 and 14718278
Volume :
3
Database :
OpenAIRE
Journal :
Molecular Ecology Notes
Accession number :
edsair.doi...........82ce88d8c95098835241b87da726ac47