MOESM2 of Old World cutaneous leishmaniasis treatment response varies depending on parasite species, geographical location and development of secondary infection

Additional file 2: Figure S1. Scheme representing the current leishmaniasis treatment policy in KSA. Figure S2. Leishmania spp. identification in Central Region by PCR-RFLP analysis of parasite ITS1 region. Lane Lt: L. tropica positive control; Lane Lm: L. major positive control; Lanes 1–10: different examples of Leishmania isolates from Rass, Dwadmi and Muzahmyyah. Figure S3. Leishmania spp identification in Al Madinah Province by PCR-RFLP analysis of parasite ITS1 region. Lanes 1–4: different examples of Leishmania isolates from Aljadaida and Sulailah, Al Madinah Province; Lanes 1 and 2: L. tropica samples; Lanes 3 and 4: L. major samples; Lane Lm: L. major positive control; Lane Lt: L. tropica positive control. Figure S4. Leishmania spp. identification in Al Ahsa Region by PCR-RFLP analysis of parasite ITS1 region. Lanes 1–13: different examples of Leishmania isolates from Al Ahsa Region; Lane Lt: L. tropica positive control; Lane Lm: L. major positive control. Figure S5. Leishmania spp identification in Asir Region by PCR-RFLP analysis of parasite ITS1 region. Lanes 1–5: different examples of Leishmania isolates from Asir Province. Figure S6. Distribution of Leishmania species (a) and patient response to anti-leishmanial treatment (b) within the Eastern region of Saudi Arabia. The map was created using software ArcGIS 10 (ESRI, Redlands, CA). Figure S7. Distribution of Leishmania species (a) and patient response to anti-leishmanial treatment (b) within the Northwest region of Saudi Arabia. The map was created using software ArcGIS 10 (ESRI, Redlands, CA). Figure S8. Distribution of Leishmania species (a) and patient response to anti-leishmanial treatment (b) within the southwest region of Saudi Arabia. The map was created using software ArcGIS 10 (ESRI, Redlands, CA).