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Determination of Pitavastatin in Human Plasma by LC–MS–MS
- Source :
- Chromatographia. 69:1041-1047
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- A simple and rapid LC–MS–MS assay was developed and validated for the quantitative determination of pitavastatin in human plasma. Sample pretreatment involved simple protein precipitation by addition of acetonitrile. Separation was on an Agilent 1.8 μm Zorbax SB-C18 column (150 mm × 4.6 mm) at 25 °C using isocratic elution with methanol–0.1% formic acid in water (85:15, v/v) at a flow rate of 0.4 mL min−1. Detection was performed using electrospray ionization in positive ion multiple reaction monitoring mode by monitoring the ion transitions m/z 422.0 → 290.1 for pitavastatin, and m/z 330.1 → 192.1 for paroxetine (IS). LC–MS–MS was found to improve the quantitation of pitavastatin in plasma and was successfully applied in pharmacokinetic studies.
- Subjects :
- Electrospray
Chromatography
Chemistry
Electrospray ionization
Organic Chemistry
Clinical Biochemistry
Selected reaction monitoring
Reversed-phase chromatography
Tandem mass spectrometry
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
medicine
Protein precipitation
Pitavastatin
medicine.drug
Subjects
Details
- ISSN :
- 16121112 and 00095893
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- Chromatographia
- Accession number :
- edsair.doi...........85db809cf1bd235010f2ca234a496fbc