Back to Search
Start Over
Osmometric Measurements of Cryoprotective Agent Permeation into Tissues
- Source :
- Cryopreservation and Freeze-Drying Protocols ISBN: 9781071607824
- Publication Year :
- 2020
- Publisher :
- Springer US, 2020.
-
Abstract
- Quantification of the amount of cryoprotective agent (CPA) in a tissue is an essential step in the design of successful cryopreservation protocols. This chapter details two inexpensive methods to measure cryoprotective agent permeation into tissues as functions of time. One of the methods to measure the CPA permeation is to permeate a series of tissue samples from a surrounding solution at a specified concentration of CPA, each sample for a different amount of time, and then to quantitate the amount of CPA that was taken up in the tissue during that time period. The quantification is performed by equilibrating the permeated tissue with a surrounding solution and then measuring the osmolality of the solution to determine the amounts of CPAs that have come out of each tissue sample corresponding to each permeation time. An alternative method to measuring the CPA permeation as a function of time, which requires fewer tissue samples, is to measure the CPA efflux as a function of time. In the efflux method, a CPA-permeated tissue sample is placed in a surrounding solution, and solution samples are taken at different time points throughout the efflux to quantitate how much CPA has left the tissue by each time point.
- Subjects :
- Alternative methods
0303 health sciences
030219 obstetrics & reproductive medicine
Chromatography
Chemistry
Tissue sample
Permeation
Cryopreservation
03 medical and health sciences
0302 clinical medicine
Osmometer
Cryoprotective Agent
cardiovascular system
heterocyclic compounds
030304 developmental biology
Subjects
Details
- ISBN :
- 978-1-07-160782-4
- ISBNs :
- 9781071607824
- Database :
- OpenAIRE
- Journal :
- Cryopreservation and Freeze-Drying Protocols ISBN: 9781071607824
- Accession number :
- edsair.doi...........8672961059b3a898e6889f6547215b6a