Back to Search
Start Over
Utility of Cerebrospinal Fluid Cell-Free DNA in Patients with EGFR-Mutant Non-Small-Cell Lung Cancer with Leptomeningeal Metastasis
- Source :
- Targeted Oncology. 16:207-214
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Leptomeningeal metastasis (LM) is a fatal complication of advanced non-small-cell lung cancer (NSCLC). The aim of this study was to evaluate the utility of cerebrospinal fluid (CSF) as a medium for epidermal growth factor receptor (EGFR) mutation testing in clinical practice. We prospectively enrolled patients with EGFR-mutant NSCLC who underwent CSF sampling for suspected LM. The supernatant of CSF after routine cytology examination was collected. The diagnosis of LM was established according to EANO-ESMO criteria. CSF and plasma cell-free DNA (cfDNA) were retrieved for EGFR mutation testing. Fifty-one patients with a median age of 62.7 years were enrolled. The median duration from initial diagnosis to CSF sampling was 23.0 months and most patients (94.1%) had received at least one EGFR-tyrosine kinase inhibitor. Adenocarcinoma cells were found in 37 CSF samples (72.5%), and 48 (94.1%) patients had confirmed or probable LM. Thirty-five of these 48 patients (72.9%) had valid EGFR mutation-testing results using CSF cfDNA and tended to have higher white blood cell counts and positive cytology in their CSF compared to those with invalid mutation testing results. The overall detection rate of EGFR mutation in CSF cfDNA was 68.8%, and the T790M detection rate was 14.6%. In 37 patients with paired CSF and plasma samples, the concordance rate of the EGFR mutation results was 29.7%. For patients with EGFR-mutant NSCLC with LM, CSF supernatant is a valuable source for EGFR mutation testing and may provide important information.
- Subjects :
- 0301 basic medicine
Cancer Research
medicine.medical_specialty
business.industry
medicine.disease
Gastroenterology
03 medical and health sciences
T790M
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Cerebrospinal fluid
Oncology
Cell-free fetal DNA
030220 oncology & carcinogenesis
White blood cell
Internal medicine
medicine
Mutation testing
Adenocarcinoma
Pharmacology (medical)
Lung cancer
Complication
business
Subjects
Details
- ISSN :
- 1776260X and 17762596
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Targeted Oncology
- Accession number :
- edsair.doi...........86990ed65619a4293d110e7c84c8c380