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Argumentation is a promising approach to handle inconsistent knowledge bases, based on the justification of plausible conclusions by arguments. Because of inconsistency, however, arguments may be defeated by counterarguments (or defeaters). The problem is thus to select the most acceptable arguments. In this paper we investigate preference-based acceptability. The basic idea is to accept undefeated arguments and also arguments that are preferred to their defeaters. We say that these arguments defend themselves against their defeaters. We define argumentation frameworks based on that preference-based acceptability. Finally, we study associated inference relations for reasoning with inconsistent knowledge bases.