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High prevalence of multidrug-resistant non-fermentative Gram-negative bacilli harboring bla and bla metallo-beta-lactamase genes in Birjand, south-east Iran
- Source :
- Iranian Journal of Microbiology.
- Publication Year :
- 2021
- Publisher :
- Knowledge E, 2021.
-
Abstract
- Background and Objectives: Non-fermentative Gram-negative Bacilli (NFGNB) is known as a major cause of health- care-associated infections with high levels of antibiotic resistance. The aim of this study was to investigate the antibiotic resistance profiles and molecular characteristics of metallo-beta-lactamase (MBL)-producing NFGNB. Materials and Methods: In this cross-sectional study, the antibiotic resistance profile of 122 clinical NFGNB isolates was determined by the Kirby-Bauer disk diffusion and microdilution broth methods. Bacterial isolates were investigated for the detection of MBLs production using the combination disk diffusion Test (CDDT). The existence of bla , bla , and bla NDM genes in all carbapenem-resistant isolates was determined employing polymerase chain reaction (PCR) assays. Results: High resistance in Pseudomonas aeruginosa was reported to cefotaxime and minocycline, whereas Acinetobacter baumannii isolates were highly resistant to all antibiotics except colistin. Multidrug resistance (MDR)-NFGNB (66% vs. 12.5%, P=0.0004) and extensively drug resistant (XDR)-NFGNB (55.7% vs. 12.5%, P=0.001) isolates were significantly more common in hospitalized patients than in outpatients. The production of MBL was seen in 40% of P. aeruginosa and 93.3% of A. baumannii isolates. It was found that 33.3% and 46.7% of carbapenem-resistant P. aeruginosa isolates, and 13.3% and 28.9% of carbapenem-resistant A. baumannii isolates were harboring bla IMP-1 and bla VIM-1 genes, respectively. The incidence of MDR (98.2% vs. 28.3%, P
- Subjects :
- Microbiology (medical)
Cefotaxime
biology
Pseudomonas aeruginosa
medicine.drug_class
Antibiotics
Drug resistance
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
medicine.disease_cause
biology.organism_classification
Microbiology
Acinetobacter baumannii
Multiple drug resistance
Antibiotic resistance
medicine
Colistin
medicine.drug
Subjects
Details
- ISSN :
- 20084447 and 20083289
- Database :
- OpenAIRE
- Journal :
- Iranian Journal of Microbiology
- Accession number :
- edsair.doi...........87fb66ea3588a1bfd83e19b537497b52