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High prevalence of multidrug-resistant non-fermentative Gram-negative bacilli harboring bla and bla metallo-beta-lactamase genes in Birjand, south-east Iran

Authors :
Ali Hashemi
Masoud Yousefi
Parvin Askari
Babak Roshanravan
Yasaman Rezaei
Mohammad Hasan Namaei
Source :
Iranian Journal of Microbiology.
Publication Year :
2021
Publisher :
Knowledge E, 2021.

Abstract

Background and Objectives: Non-fermentative Gram-negative Bacilli (NFGNB) is known as a major cause of health- care-associated infections with high levels of antibiotic resistance. The aim of this study was to investigate the antibiotic resistance profiles and molecular characteristics of metallo-beta-lactamase (MBL)-producing NFGNB. Materials and Methods: In this cross-sectional study, the antibiotic resistance profile of 122 clinical NFGNB isolates was determined by the Kirby-Bauer disk diffusion and microdilution broth methods. Bacterial isolates were investigated for the detection of MBLs production using the combination disk diffusion Test (CDDT). The existence of bla , bla , and bla NDM genes in all carbapenem-resistant isolates was determined employing polymerase chain reaction (PCR) assays. Results: High resistance in Pseudomonas aeruginosa was reported to cefotaxime and minocycline, whereas Acinetobacter baumannii isolates were highly resistant to all antibiotics except colistin. Multidrug resistance (MDR)-NFGNB (66% vs. 12.5%, P=0.0004) and extensively drug resistant (XDR)-NFGNB (55.7% vs. 12.5%, P=0.001) isolates were significantly more common in hospitalized patients than in outpatients. The production of MBL was seen in 40% of P. aeruginosa and 93.3% of A. baumannii isolates. It was found that 33.3% and 46.7% of carbapenem-resistant P. aeruginosa isolates, and 13.3% and 28.9% of carbapenem-resistant A. baumannii isolates were harboring bla IMP-1 and bla VIM-1 genes, respectively. The incidence of MDR (98.2% vs. 28.3%, P

Details

ISSN :
20084447 and 20083289
Database :
OpenAIRE
Journal :
Iranian Journal of Microbiology
Accession number :
edsair.doi...........87fb66ea3588a1bfd83e19b537497b52