Hericinoids A‒C, cyathane diterpenoids from culture of mushroom Hericium erinaceus

Three previously undescribed cyathane-type diterpenoids, hericinoids A-C (1‒3) and three known analogues (4‒6) have been isolated from fermentation broth of Hericium erinaceus. Their structures of hericinoids A-C were determined by in-depth analyses of NMR spectra and HRESIMS. The absolute configurations of compounds 1 and 2 were determined by the ROESY spectra along with the DP4+ calculations based on quantum chemistry. Compounds 1–5 were inactive on the enhancement of NGF-induced neutrite outgrowth in PC-12 cells. Instead, compounds 2, 4, and 5 showed potent cytotoxicity against HL-60 cell lines with the IC50 values of 18.3, 8.9, and 0.5 μM, respectively. Compounds 4 and 5 showed moderate cytotoxicities against MCF-7 cell lines with the IC50 values ranging from 13.4 to 15.8 μM.