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Escherichia coli σ70 and NusA proteins
- Source :
- Journal of Molecular Biology. 220:325-333
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- In this paper we examine the physical properties and potential for self-association of the Escherichia coli transcription factors, σ 70 and NusA. We show, by a combination of chemical crosslinking, equilibrium and velocity sedimentation, quasi-elastic light scattering, and small-angle X-ray scattering that NusA exists as a monomer at KCl concentrations between 0.01 and 1.5 m , and that σ 70 exists as a monomer at KCl concentrations between 0.1 and 1.5 m . The shape and hydration characteristics of each of these monomeric proteins are also examined. The results serve as background for the companion paper in which a thermodynamic analysis is made of the interactions of these transcription factors with E. coli core RNA polymerase in solution and as a component of the functional transcription complex.
Details
- ISSN :
- 00222836
- Volume :
- 220
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Biology
- Accession number :
- edsair.doi...........8aefdec6126d3648d1f02d119bfd3ffc