Measurements of diel variation of acoustic backscatter power from phytoplankton

Phytoplankton is a primary producer in the ocean, and its vegetative activity plays an important role in controlling the global environment. In this study, the high-frequency acoustic signals were collected to evaluate the photosynthetic activity of phytoplankton during a day. The integrated backscatter power (IBP) from a dinoflagellate Cochlodinium polykrikoides was measured by a 5 MHz acoustic system during a 5-day cultivation period with a 14 h:10 h light:dark cycle. IBP increased by 0.6 dB in five days, but varied by 0.83 ± 0.1 dB during an irradiance cycle. The daily increase in IBP was a result of an increase in the number of cells during cultivation, while the diel variation was partly resulted from the variation of volume of cells by photosynthesis. In addition, cell division and separation might affect IBP. IBP of another dinoflagellate Amphidinium carterae Hulburt was also measured by a 10MHz transducer during a 3-day cultivation period while cell volume and photosynthetic capacity were measured four times a day (07:00, 12:00, 19:00, 24:00). This study suggests that high-frequency acoustics may be a meaningful tool to investigate the photosynthetic metabolism of a phytoplankton cell. [This work was supported by Defense Acquisition Program Administration and Agency for Defense Development under the contract UD130007DD.]