Back to Search
Start Over
Doubly derivatized poly(lactide)–albumin nanoparticles as blood vessel-targeted transport device for magnetic resonance imaging (MRI)
- Source :
- Journal of Nanoparticle Research. 23
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Molecular imaging using magnetic resonance imaging (MRI) is expected to play a crucial future role in oncological diagnosis and in monitoring of therapeutic progress. Targeted nanoparticle contrast media (CM) with high relaxivities are required in order to obtain adequate signal-to-noise ratios as well as visualization of a desired pathologic area of the human body. The aims of this study were to synthesize and define certain physicochemical and enhancement properties of new doubly derivatized polylactic acid–bovine serum albumin (PLA-BSA) nanoparticles (NPs) modified by the covalent coupling of glutaraldehyde as a crosslinking agent. An additional functionalization with endothelial cells (ECs) targeting groups (tomato lectins; LEA) and signal-emitting moieties (DTPA-Gd) enables its use as a macromolecular, biodegradable contrast agent for MRI. The NPs were characterized by different spectroscopies, size exclusion chromatography, and scanning and transmission electron microscopy. In a human vein model, the dynamics of the nanoparticle interactions with the vein wall were examined in MRI, with correlative imaging in electron microscopy. In vitro studies were conducted to show endothelial binding and persistent enhancement at the apical EC surface. NPs with a diameter between 55 and 75 nm, able to carry simultaneous signal emitting, and targeting motifs on a single construct were successfully prepared. A high Gd payload and endothelial binding to blood vessel walls were observed. The binding affinity and specificity of LEA was preserved, and a strong enhancement at the endothelium was achieved. The stabilized core–shell structure of PLA-NP might allow for further encapsulation of lipophilic drugs or for attachment of other targeting molecules, such as antibodies. Graphical abstract
- Subjects :
- Materials science
Size-exclusion chromatography
Serum albumin
Nanoparticle
Bioengineering
02 engineering and technology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
General Materials Science
biology
General Chemistry
021001 nanoscience & nanotechnology
Condensed Matter Physics
Atomic and Molecular Physics, and Optics
chemistry
Covalent bond
Modeling and Simulation
biology.protein
Biophysics
Surface modification
Glutaraldehyde
Molecular imaging
0210 nano-technology
030217 neurology & neurosurgery
Macromolecule
Subjects
Details
- ISSN :
- 1572896X and 13880764
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Journal of Nanoparticle Research
- Accession number :
- edsair.doi...........8cf9ee10f9a721907c2159f02da2ed3b
- Full Text :
- https://doi.org/10.1007/s11051-021-05157-w