AAVS1 Knock-in v1

Authors :: Dirk Hockemeyer
Publication Year :: 2022
Publisher :: ZappyLab, Inc., 2022.
Abstract: This protocol describes the standard procedure to knock-in constructs to the AAVS1 safe harbor locus in hPSCs. General Notes: 1. The AAVS1 knock-in construct, AAVS1-SA-neo-CAGGS-PE2-2A-GFP, can be found at AddGene (Catalog: 180014, RRID:Addgene_180014) 2. Throughout this protocol, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs.