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Multivalent Antigen Display on Nanoparticles Diversifies B Cell Responses
- Publication Year :
- 2026
- Publisher :
- Zenodo, 2026.
-
Abstract
- Nanoparticles for multivalent display and delivery of vaccine antigens have emerged as a promising avenue for enhancing B cell responses to protein subunit vaccines. Here, we evaluated B cell responses in rhesus macaques immunized with prefusion stabilized Respiratory Syncytial Virus (RSV) F glycoprotein trimer compared to nanoparticles displaying 10 or 20 copies of the same antigen. We show that multivalent display skews antibody specificities and drives epitope-focusing of responding B cells. Antibody cloning and repertoire sequencing revealed that focusing was driven by expansion of clonally distinct B cells through recruitment of diverse precursors. We identified two antibody lineages that developed either ultrapotent neutralization or pneumovirus cross-neutralization from precursor B cells with low initial affinity for the RSV-F immunogen. This suggests that increased avidity by multivalent display facilitates the activation and recruitment of these cells. Diversification of the B cell response by multivalent nanoparticle immunogens has broad implications for vaccine design.
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........8e5c8c6f65335ccc060c0bcc37b3b1fb
- Full Text :
- https://doi.org/10.5281/zenodo.7895251