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Viroid Elimination by Thermotherapy, Cold Therapy, Tissue Culture, In Vitro Micrografting, or Cryotherapy
- Publication Year :
- 2017
- Publisher :
- Elsevier, 2017.
-
Abstract
- Viroids cannot be controlled by therapeutic treatments in fields, orchards, vineyards, or palm plantations. Hence, the elimination of viroids from infected plants has been a challenging issue and different approaches have been studied to produce viroid-free planting material. This chapter describes viroid elimination by thermotherapy, cold therapy, tissue culture, in vitro micrografting, or cryotherapy to obtain viroid-free materials. Elevated temperatures do not inactivate or eliminate most viroids from their host plants, whereas cold therapy can be effective depending on the host–viroid system. Meristem culture and shoot-tip micrografting are good methods to obtain viroid-free plants: the eradication rate depends on the size of the shoot, the viroid or its variant, and the host and its variety. The combination of thermotherapy plus shoot-tip culture is more effective for viroid elimination. Cryotherapy of shoot tips, an innovative technique for pathogen eradication based on cryopreservation, did not result in viroid elimination. Further experiments on other host–viroid systems and on combination of cold therapy and cryotherapy are under evaluation.
- Subjects :
- 0106 biological sciences
Viroid
viruses
medicine.medical_treatment
fungi
food and beverages
Cryotherapy
04 agricultural and veterinary sciences
Biology
biology.organism_classification
01 natural sciences
Virology
Cryopreservation
In vitro
040501 horticulture
Tissue culture
Cold Therapy
medicine
Host plants
0405 other agricultural sciences
010606 plant biology & botany
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........8f0d05c72118ba66ad65755037c50039