SPECTROPHOTOMETRYC DETERMINATION OF SERTRALINE IN PURE AND BLOOD SAMPLE

A simple and sensitive spectrophotomteric method has been developed for the determination of Sertraline in pharmaceutical and blood sample. The current method is depend upon the reaction between the sertraline and chloranilic acid in slightly alkaline medium, giving a purple colour complex having maximum absorbance at 527.5 nm. The reaction is selective for sertraline with 0.01mg/mL.The colour reaction obeys Beer’s law from 0.1mg to 5mg/mL of sertraline and relative standard deviation is 0.19%. The quantitative estimation of sertraline in blood sample is also studied. Key Words: Spectrophotometric, Sertraline, chloranilic acid, Beer’s law, quantitative estimation . e-mail: asif_hanif101@yahoo.com INTRODUCTION Sertraline Hydrochloride is a Selective Serotonin Reuptake Inhibitor (SSRI) and is a tricyclic compound with antidepressant activity 1 . The antidepressant effect of sertraline is presumed to be linked to its ability to inhibit the neuronal reuptake of serotonin 2 . It has only very weak effects on norepinephrine and dopamine neuronal reuptake. At clinical doses, sertraline blocks the uptake of serotonin into human platelets