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Molecular phylogenetic study of Pinus in Korea based on chloroplast DNA psbA-trnH and atpF-H sequences data

Authors :
Jong-Cheol Yang
Joo-Hwan Kim
You-Mi Lee
Jeong-Ki Hong
Source :
Korean Journal of Plant Taxonomy. 44:111-118
Publication Year :
2014
Publisher :
The Korean Society of Plant Taxonomists, 2014.

Abstract

This study aims to define the phylogenetic relationship within Korean Pinus L. and to find themolecular markers which resolve the phylogenetic relationship in genus Pinus. cpDNA atpF-H and psbA-trnHregions were used as molecular markers. We performed the molecular phylogenetic study on 17 taxa of Pinus inKorea. The combined analyses of two gene loci showed that Korean Pinus was a monophyletic group supportedby 100% BP. According to the results of separate analyses, psbA-trnH region seems to work better resolvingpower to clarify the phylogenetic ambiguity in Korean Pinus than those of atpF-H region. Also, we tried tochecked the value and resolution of two chloroplast DNA loci on phylogenetic implications. Keywords: Pinus, subgenus Diploxylon & Haploxylon, atpF-H, psbA-trnH 적 DNA 요: 엽록체 atpF-H, psbA-trnH region을 마커로활용하여국내에분포하는 소나무속 식물들 중 17분류군에 대한 분자계통학적 연구를 수행하여 한국산 소나무속의 계통학적 유연관계를 규명하고, 소나무속의유연관계를 잘 나타낼 수 있는 분자마커를 찾아내고자 연구가 수행되었다. atpF-H, psbA-trnH region의 조합분석결과 한국산 소나무속은 100%의 BP로 지지되는 단계통군으로 확인되었으며, 소나무아속과 잣나무아속으로 명확히 구분되어졌다. 본 연구에서 이용된 두 개의 분자마커 중 psbA-trnH region이 atpF-H region보다한국산 소나무속의 계통 및 유연관계를 규명하는데 다소 높은 해상력을 나타내었다.

Details

ISSN :
12258318
Volume :
44
Database :
OpenAIRE
Journal :
Korean Journal of Plant Taxonomy
Accession number :
edsair.doi...........90eb6e2e1c254f115990bee8b912fa5b