PM373 In Vivo Assessment of Biodistribution and Efficacy of Bone Marrow Mononuclear Cells for Ischaemia-mediated Neovascularisation

Withdrawn GHEART Vol 9/1S/2014 j March, 2014 j POSTER/2014 WCC Posters PM375 Endothelin-1 Differentially Activates Protein Kinase C and Rho Kinase in Large and Small Arteries Kanchani Rajopadhyaya, Jessica Maddern, John Beltrame, David Wilson, Amenah Jaghoori* Medicine, The University of Adelaide, Vascular Diseases and Therapeutics Reserach Group, The Basil Hetzel Institute For Medical Research, Physiology, The University of Adelaide, Cardiology, The Queen Elizabeth Hospital, Adelaide, Australia Introduction: The clinical management of vasospastic disorders is focused on the blockade of extracellular Ca entry and or specific G-protein coupled receptors. However these therapies neglect numerous additional modes of Ca entry and often do not resolve small vessel dysfunction. This warrants investigating whether there are fundamental differences in the ways that large and small arteries constrict to a given agonist. Objectives: This study aimed to identify whether protein kinase C (PKC) and Rho kinase (ROK)-mediated Ca sensitisation is differentially regulated in large vs small vessels in an acute endothelin-1 (ET-1) model. Methods: Using wire myography, the vascular response to ET-1 was determined acutely in rat caudal arteries following incubation with pharmacological inhibitors. Results: In large caudal arteries (500mm), PKC inhibition prior to ET-1 attenuated the sustained phase of ET-1-mediated vasoconstriction (p 0.05) but did attenuate constriction in the large caudal artery (p