Flavonoids with Hepatoprotective Activity from the Leaves of Cleome chelidonii

One new flavonol glycoside, named cleomeside C (1), together with eight known flavonol glycosides (2–9) were isolated by various chromatography methods from the leaves of Cleome chelidonii. Their structures were elucidated by IR, UV, HR-ESI-MS, NMR (1D & 2D) experiments. Compounds 2–8 were isolated for the first time from the genus Cleome. The cytotoxicity and hepatoprotective activity in HepG2 human hepatoma cell line of compounds (1–3 and 6) were measured by MTT assay. At the concentration of 25 μM and 50 μM, compound 2 as well as compound 3 at 25 μM showed cytotoxic activity (cell viability was decreased 21.7%, 31.8% and 21.8%, respectively, compared to control). Compounds 2 and 6 expressed hepatoprotective effect at the concentration of 100 μM with prevention percentage of 32.3% and 34.3%, respectively, compared to that of 80.3% of quercetin positive control).