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SYBR® Green qPCR Screening Methods for Detection of Anti-herbicide Genes in Genetically Modified Processed Products
- Source :
- Journal of Northeast Agricultural University (English Edition). 23:57-64
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The use of genetically modified organisms (GMOs) as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize GMOs as food or food ingredients. Thus, analytical methods for detection of GMOs are necessary in order to verify compliance with labelling requirements. There are few effective screening methods for processed GM (genetically modified) products. Three anti-herbicide genes (CP4-EPSPS, BAR and PAT) are common exogenous genes used in commercialized transgenic soybean, maize and rice. In the present study, a new SYBR® Green qPCR screening method was developed to simultaneously detect the three exogenous anti-herbicide genes and one endogenous gene in a run. We tested seven samples of representative processed products (soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, maize protein powder, maize starch, and maize jam) using the developed method, and amplicons of endogenous gene and transgenic fragments were obtained from all the processed products, and the sensitivity was 0.1%. These results indicated that SYBR® Green qPCR screening method was appropriate for qualitative detection of transgenic soybean, maize and rice in processed products.
- Subjects :
- business.industry
Transgene
010401 analytical chemistry
food and beverages
04 agricultural and veterinary sciences
Biology
Amplicon
040401 food science
01 natural sciences
Maize starch
0104 chemical sciences
Genetically modified organism
Biotechnology
0404 agricultural biotechnology
Labelling
media_common.cataloged_instance
European union
business
Gene
Rice cereal
media_common
Subjects
Details
- ISSN :
- 10068104
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Journal of Northeast Agricultural University (English Edition)
- Accession number :
- edsair.doi...........928f96d9da74fb95d2331ab5bdf9e5ea